Identification of Nocardia farcinica by a PCR primer amplifying a specific DNA band for the bacterium.

نویسندگان

  • Taichi Hasegawa
  • Tohru Gonoi
  • Junji Ito
  • Takahisa Kogure
  • Katsukiyo Yazawa
  • Yuzuru Mikami
چکیده

A PCR primer specific to Nocardia farcinica was prepared based on sequence information of random amplified polymorphic DNA (RAPD) analysis. The PCR primer amplifies N. farcinica species only; no amplification was observed in 25 other Nocardia strains that we tested. Specificity of the primer for N. farcinica was also confirmed using other fungal and bacterial strains that are frequently isolated from clinical samples such as sputa and broncho alveolar lavage (VAL).

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عنوان ژورنال:
  • Nihon Ishinkin Gakkai zasshi = Japanese journal of medical mycology

دوره 48 4  شماره 

صفحات  -

تاریخ انتشار 2007